dc.contributor.advisor | Gómez Jiménez, Martha | |
dc.contributor.advisor | Sánchez Mora, Ruth Mélida | |
dc.contributor.author | Solano Meneses, Dayana | |
dc.contributor.author | Marmolejo González, Everth David | |
dc.date.accessioned | 2022-10-20T20:08:27Z | |
dc.date.available | 2022-10-20T20:08:27Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5721 | |
dc.description.abstract | Al ser una puerta de entrada de diferentes agentes biológicos, el sistema digestivo alberga gran cantidad de
bacterias, correspondiente aproximadamente a un 90% de la flora general del intestino, considerándose uno de
los lugares en donde estos microorganismos tienen mayor impacto; de hecho, se ha visto que dicha influencia
tiene un efecto positivo en otros sistemas al intervenir en procesos metabólicos, de estimulación celular o
protección a infecciones, por lo que en la actualidad esté sistema es considerado un blanco terapéutico
prometedor para diversas enfermedades y garantizar su estabilidad es un reto para los investigadores.
Con respecto a esto, los probióticos resultan ser una herramienta efectiva para el mantenimiento de este sistema,
ya que contribuyen a la estabilización y recuperación de la flora intestinal, siendo Lactobacillus uno de los
géneros con mayor cantidad de cepas implementadas. A pesar de esto y de su potencial como herramienta
terapéutica, requieren de mayores estudios que esclarezcan los mecanismos por los cuales actúan, lo que puede
realizarse por medio de la exposición de modelos biológicos de estudio a diferentes cepas de este género (1).
Por lo anterior, en el presente estudio se evaluaron dos cepas de Lactobacillus, usando Caenorhabditis elegans
como modelo de estudio por sus características morfológicas a nivel intestinal, ventajas en el costo de
mantenimiento y facilidad de monitoreo, con el fin de ampliar los conocimientos ante el microorganismo
probiótico y proponer al mismo como herramienta terapéutica ante patologías relacionadas con el eje intestinal | spa |
dc.description.tableofcontents | Índice de figuras
Índice de gráficas
Índice de tablas
RESUMEN
INTRODUCCIÓN
OBJETIVOS
OBJETIVO GENERAL
OBJETIVOS ESPECÍFICOS
1. ANTECEDENTES
2. MARCO REFERENCIAL
2.1. Probióticos
2.2. Selección de probióticos
2.3. Lactobacillus spp uno de los principales géneros probióticos
2.4. Viabilidad y probióticos
2.5. Modelos para el estudio de probióticos
2.5.1. Caenorhabditis elegans como modelo de estudio en diferentes enfermedades.
3. DISEÑO METODOLÓGICO
3.1. Cultivo y mantenimiento del nematodo
3.2. Cultivo y mantenimiento de bacterias probióticas
3.3. Sincronización del nematodo
3.4. Determinación de la concentración ideal del probiótico
3.5. Ensayo de preferencia alimentaria
3.6. Determinación de la vida útil del nematodo frente al tratamiento probiótico
3.7. Observación de cambios a nivel intestinal del nematodo frente al probiótico
3.8. Análisis estadístico
4. RESULTADOS
4.1. Identificación de los estadios larvarios de C. elegans
4.2. Concentración óptima de L. rhamnosus y L. plantarum incorporados en la dieta de C. elegans
4.3. Preferencia de C. elegans por el estado de la dieta
4.4. Longevidad de C. elegans tras el consumo de concentraciones seleccionadas del probiótico
4.5. Supervivencia al estrés térmico tras el consumo de L. rhamnosus y L. plantarum.
4.6. Evaluación cualitativa de la grasa intestinal en C. elegans tras el consumo de L. rhamnosus y L.
plantarum.
5. DISCUSIÓN
6. CONCLUSIONES
7. REFERENCIAS BIBLIOGRÁFICAS
8. ANEXOS | spa |
dc.format.extent | 46p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.rights | Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Evaluación de los efectos intestinales y cambios en la longevidad del nematodo Caenorhabditis elegans cepa mutante AX1410 y cepa silvestre N2 expuestas a la acción de Lactobacillus rhamnosus y Lactobacillus plantarum | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | spa |
dc.publisher.faculty | Universidad colegio mayor de Cundinamarca | spa |
dc.publisher.program | Bacteriología y Laboratorio Clínico | spa |
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dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.proposal | Probióticos | spa |
dc.subject.proposal | Modelo de estudio | spa |
dc.subject.proposal | Sistema digestivo | spa |
dc.subject.proposal | Herramientas terapéuticas | eng |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |