Apoptosis de neutrófilos mediada por anticuerpos dirigidos contra péptidos derivados de proteínas de mycobacterium tuberculosis h37rv

Alfonso Alfonso, Angie Paola

dc.contributor.advisor	Carabalí Isajar, Mary Lilián
dc.contributor.advisor	Castro Molina, Susan Lorena
dc.contributor.author	Alfonso Alfonso, Angie Paola
dc.date.accessioned	2021-06-30T15:02:04Z
dc.date.available	2021-06-30T15:02:04Z
dc.date.issued	2019-11
dc.identifier.uri	https://repositorio.unicolmayor.edu.co/handle/unicolmayor/307
dc.description.abstract	Los neutrófilos son células de la inmunidad innata que predominan en pacientes con tuberculosis activa, estos polimorfonucleares mediante la apoptosis, pueden contribuir en la resolución de la infección sin ocasionar daño en el tejido pulmonar, favorecer la presentación antigénica e inducir la producción de citoquinas proinflamatorias, para contrarrestar el crecimiento micobacteriano; sin embargo, los factores de patogenicidad y virulencia que posee Mycobacterium tuberculosis, pueden producir otras formas de muerte celular que ocasionan daño tisular y favorecen la inflamación exacerbada. La apoptosis puede ser inducida por fagocitosis que se lleva a cabo cuando los antígenos se encuentran unidos a anticuerpos (opsonofagocitosis), este fenómeno no se ha descrito en procesos infecciosos relacionados con Mycobacterium y considerando la importancia de este tipo de muerte celular como parte de una respuesta inmune protectiva, es necesario evaluar anticuerpos que promuevan este tipo de muerte progamada. En este sentido, se planteó el siguiente trabajo, evaluar si la opsonofagocitosis con IgGs que reconocen antígenos de la micobacteria, puede mediar la apoptosis de neutrófilos, por consiguiente, se seleccionaron y aislaron inmunoglobulinas G, que reconocieron 9 secuencias peptídicas presentes en diferentes proteínas de la envoltura de Mycobacterium tuberculosis H37Rv. Se encontró que la opsonofagocitosis con los anticuerpos péptidos-específicos 37964, 37765, 31107, 16300 y 38373 aumentaron la apoptosis en los polimorfonucleares, lo cual podría indicar que algunas inmunoglobulinas, posiblemente pueden mediar la apoptosis en estas células, de forma que los péptidos que son reconocidos y para los cuales se aislaron las IgGs, probablemente pueden favorecer de alguna forma la generación de una respuesta inmune protectiva.	spa
dc.description.tableofcontents	1. Resumen 13 2. Introducción 14 3. Objetivos 16 3.1. Objetivo general 16 3.2. Objetivos específicos 16 4. Antecedentes 17 5. Marco Teórico 19 5.1. Generalidades de la tuberculosis 19 5.2. Mycobacterium tuberculosis y el complejo MTB 20 5.3. Epidemiología de la tuberculosis 21 5.4. Diagnóstico de la tuberculosis 23 5.4.1. Criterio bacteriológico 24 5.4.2. Criterio histopatológico 24 5.4.3. Criterio tuberculínico y prueba QuantiFERON 25 5.5. Tratamiento para la TB 25 5.6. Respuesta inmune innata ante Mycobacterium tuberculosis 26 5.6.1. Células epiteliales alveolares 27 5.6.2. Macrófagos 28 5.6.3. Células dendríticas (DCs) 29 5.6.4. Células asesinas naturales (NKs) 29 5.6.5. Neutrófilos 30 5.7. Respuesta inmune adaptativa ante Mycobacterium tuberculosis 31 5.7.1. Linfocitos T (LT) 31 5.7.2. Linfocitos B (LB) 32 5.8. Desarrollo de vacunas contra la tuberculosis 33 6. Metodología 35 6.1. Diseño metodológico 35 6.1.1. Tipo de investigación 35 6.1.2. Universo 35 6.1.3. Población 35 6.1.4. Muestra 35 6.2. Técnicas y procedimientos 36 6.2.1. Selección de péptidos 36 6.2.1.1. Aproximación bioinformática 36 6.2.1.2. Aproximación experimental 37 6.2.1.2.1. Obtención de sueros de Donantes 37 6.2.1.2.2. Búsqueda de anticuerpos IgGs afines a péptidos con epítopes B 37 6.2.2. Obtención de plasmas para purificación de inmunoglobulina G que reconocen péptidos seleccionados 38 6.2.2.1. Purificación de IgG 38 6.2.2.2. Purificación de IgG péptido-específica 38 6.2.3. Ensayos de fagocitosis en neutrófilos mediada por anticuerpos 39 6.2.3.1. Aislamiento de neutrófilos de sangre periférica 39 6.2.3.2. Ensayo de opsonofagocitosis 40 6.2.3.2.1. Cultivo de Mtb H37Rv 40 6.2.3.2.2. Opsonofagocitosis de Mtb H37Rv 40 6.2.4. Procesamiento y análisis de datos 41 7. Resultados 42 7.1. Selección de péptidos provenientes de proteínas de la envoltura de Mtb H37Rv 42 7.2. Purificación de inmunoglobulina G que reconocen péptidos específicos 45 7.3. Apoptosis de neutrófilos 47 8. Discusión 53 9. Conclusiones 57 10. Recomendaciones 58 11. Referencias 59 12. Anexos 66	spa
dc.format.extent	67p.	spa
dc.format.mimetype	application/pdf	spa
dc.language.iso	spa	spa
dc.publisher	Universidad Colegio Mayor de Cundinamarca	spa
dc.relation.ispartof	No objeto asociado
dc.rights	Derechos Reservados -Universidad Colegio Myor de Cundinamarca ,2019	eng
dc.rights.uri	https://creativecommons.org/licenses/by-nc-sa/4.0/	spa
dc.title	Apoptosis de neutrófilos mediada por anticuerpos dirigidos contra péptidos derivados de proteínas de mycobacterium tuberculosis h37rv	spa
dc.type	Trabajo de grado - Pregrado	spa
dc.contributor.corporatename	Universidad Colegio Mayor de Cundinamarca	spa
dc.contributor.researchgroup	Trabajo de grado	spa
dc.coverage.country	Colombia
dc.description.degreelevel	Pregrado	spa
dc.description.degreename	Bacteriólogo(a) y Laboratorista Clínico	spa
dc.description.researcharea	Trabajo de grado	spa
dc.identifier.barcode	60186
dc.publisher.faculty	Facultad de Ciencias de la Salud	spa
dc.publisher.place	Bogotá, Distrito Capital	spa
dc.publisher.program	Bacteriología y Laboratorio Clínico	spa
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dc.rights.accessrights	info:eu-repo/semantics/openAccess	spa
dc.rights.creativecommons	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)	spa
dc.subject.lemb	Enfermedades micobacterianas
dc.subject.lemb	Epidemiología
dc.subject.lemb	Tuberculosis pulmonar
dc.subject.proposal	Tuberculosis	spa
dc.subject.proposal	Mycobacterium tuberculosis	spa
dc.subject.proposal	Neutrófilos	spa
dc.subject.proposal	Anticuerpos	spa
dc.subject.proposal	Apoptosis.	spa
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dc.type.driver	info:eu-repo/semantics/bachelorThesis	spa
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