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Caracterización in silico de los genes de virulencia PhoP -PhoQ en cepas de Pseudomonas aeruginosa fenotipo multidrogo-resistente (MDR)

dc.contributor.advisorRomero Calderón, Ibeth Cristina
dc.contributor.authorRodríguez Vásquez, Laura Ximena
dc.date.accessioned2022-03-08T20:42:42Z
dc.date.available2022-03-08T20:42:42Z
dc.date.issued2021-09-03
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/4788
dc.description.abstractPseudomonas aeruginosa es un microorganismo que presenta resistencia en los entornos clínicos. Son diferentes los mecanismos por los cuales logra evadir los antimicrobianos, uno de ellos son los sistemas de dos componentes. PhoQ y PhoP son un sistema de dos componentes conocido principalmente en Salmonella sp., es por esto que su caracterización in silico en Pseudomonas aeruginosa aporta al conocimiento sobre la identificación de nuevos marcadores moleculares asociados a virulencia y resistencia. En el presente proyecto se caracterizaron los genes PhoQ-PhoP de dos cepas MDR de P. aeruginosa haciendo uso de herramientas bioinformáticas con el objetivo de buscar mutaciones frente a las cepas sensibles a medicamentos, realizar un análisis filogenético con ortólogos y determinar características de las proteínas codificadas por estos genes. Se logró observar una mutación de cambio de sentido en PhoQ con un cambio de tirosina por fenilalanina, un distanciamiento filogenético de P. aeruginosa en comparación a ortólogos de estos genes por las diferencias funcionales y ambientales de las distintas especies y se obtuvo modelos tridimensionales de buena calidad lo cual permite realizar la búsqueda de compuestos que tengan afinidad de unión con estas proteínas, paso principal en el diseño racional de nuevos medicamentos. Estos resultados pueden ser usados a futuro para el desarrollo de posibles blancos terapéuticos o para la inhibición selectiva de P. aeruginosa en alternativas terapéuticasspa
dc.description.tableofcontentsResumen 9 Introducción 11 1. Objetivos 13 1.1 Objetivo general 13 1.2 Objetivos específicos 13 2. Antecedentes 14 3. Bases legales 16 4. Marco teórico 17 4.1 Características generales de Pseudomonas aeruginosa 17 4.2 Enfermedades o infecciones ocasionadas por Pseudomonas aeruginosa 17 4.2.1 Modo de transmisión 18 4.2.2 Tratamiento y resistencia en P. aeruginosa 18 4.2.3 Alternativas terapéuticas 19 4.3 Patogenicidad y virulencia de Pseudomonas aeruginosa 20 4.3.1 Genes de virulencia y resistencia en Pseudomonas aeruginosa 21 4.4 Función biológica de PhoP y PhoQ en microorganismos 21 4.5 Importancia de los estudios in silico en resistencia bacteriana 24 5. Metodología 25 6. Resultados 29 7. Discusión 47 8. Conclusiones 51 Referencias bibliográficas 51eng
dc.format.extent68p.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.rightsDerechos Reservados - Universidad Colegio Mayor de Cundinamarcaeng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleCaracterización in silico de los genes de virulencia PhoP -PhoQ en cepas de Pseudomonas aeruginosa fenotipo multidrogo-resistente (MDR)spa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogota D.Cspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.lembPhoQ
dc.subject.lembMDR
dc.subject.lembsensibilidad
dc.subject.proposalPseudomonas aeruginosaspa
dc.subject.proposalSistema de dos componentesspa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.rights.coarhttp://purl.org/coar/access_right/c_14cbspa


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