dc.contributor.advisor	Villamil, Luisa Marcela
dc.contributor.advisor	Castro, Susan Lorena
dc.contributor.author	Cuervo Vargas, Carla
dc.date.accessioned	2022-05-10T00:11:48Z
dc.date.available	2022-05-10T00:11:48Z
dc.date.issued	2021-11
dc.identifier.uri	https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5548
dc.description.abstract	Los probióticos son empleados cada vez más en acuicultura debido a que influyen positivamente en el crecimiento de los peces, además de mejorar sus actividades metabólicas e influir en el control de patógenos. Sin embargo, para que un microorganismo sea considerado como probiótico debe estar libre de factores de virulencia, en el caso de cepas de Bacillus, se espera que estén libres de enterotoxinas (nheA, nheB, nheC, hblA, hblC, hblD, cytK y entFM) y cereulida sintasa (ces), además de carecer de genes que puedan conferir resistencia antibiótica. Por lo tanto, se evaluó la bioseguridad de una cepa de Priestia megaterium M4, mediante la secuenciación del genoma completo, posteriormente se realizó la anotación en la base de datos de factores de virulencia (VFDB) y ResFinder. En el presente estudio no se identificaron genes asociados a virulencia ni la presencia de genes de resistencia antibiótica adquiridos. Teniendo en cuenta los resultados obtenidos, se considera que la cepa de Priestia megaterium M4 es una posible candidata para emplearse como probiótico en el cultivo de tilapia nilótica.	spa
dc.description.abstract	Probiotics are increasingly used in aquaculture because they positively influence fish growth, improve their metabolic activities and influence the control of pathogens. However, a microorganism must be free of pathogenic factors to be recognized as a probiotic. In the case of Bacillus strains, enterotoxins (nheA, nheB, nheC, hblA, hblC, hblD, cytK y entFM) and cereulide synthase (ces), in addition to antibiotic resistance genes must be absent. The objective of the present study was to evaluate the biosafety of a Bacillus megaterium M4 strain by sequencing the complete genome, followed by annotation in the virulence factor database (VFDB) and ResFinder. In the current study, virulence-associated genes and resistance-acquired genes were not identified neither of them affected the biosafety of the strain, nor were any of the genes associated with antibiotic resistance. Considering the results obtained, the Priestia megaterium M4 strain is considered a possible candidate for use as a probiotic for Nile tilapia culture.	eng
dc.format.extent	22p.	spa
dc.format.mimetype	application/pdf	spa
dc.language.iso	spa	spa
dc.publisher	Universidad Colegio Mayor de Cundinamarca	spa
dc.rights	Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2021	spa
dc.rights.uri	https://creativecommons.org/licenses/by-nc-sa/4.0/	spa
dc.title	Análisis in sillico de la bioseguridad de una cepa de Priestia sp. aislada de tilapia nilótica (Oreochromis niloticus) y su potencial uso como probiótico en acuicultura	spa
dc.type	Trabajo de grado - Pregrado	spa
dc.description.degreelevel	Pregrado	spa
dc.description.degreename	Bacteriólogo(a) y Laboratorista Clínico	spa
dc.publisher.faculty	Facultad de Ciencias de la Salud	spa
dc.publisher.place	Bogotá	spa
dc.publisher.program	Bacteriología y Laboratorio Clínico	spa
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dc.rights.accessrights	info:eu-repo/semantics/closedAccess	spa
dc.rights.creativecommons	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)	spa
dc.subject.lemb	factores de virulencia
dc.subject.lemb	resistencia antibiótica
dc.subject.proposal	Priestia megaterium	spa
dc.subject.proposal	probiótico	spa
dc.subject.proposal	bioseguridad	spa
dc.type.coar	http://purl.org/coar/resource_type/c_7a1f	spa
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85	spa
dc.type.content	Text	spa
dc.type.driver	info:eu-repo/semantics/bachelorThesis	spa
dc.type.redcol	https://purl.org/redcol/resource_type/TP	spa
dc.type.version	info:eu-repo/semantics/publishedVersion	spa
dc.rights.coar	http://purl.org/coar/access_right/c_14cb	spa

Análisis in sillico de la bioseguridad de una cepa de Priestia sp. aislada de tilapia nilótica (Oreochromis niloticus) y su potencial uso como probiótico en acuicultura

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