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Identificación de Cryptosporidium spp y Giardia duodenalis a través de Inmunofluorescencia directa (IFD) en pequeños rumiantes y aspectos epidemiológicos en granjas entre los 1300 y 2500 m.s.n.m

dc.contributor.advisorVargas Duarte, Jimmy Jolman
dc.contributor.advisorPáez Díaz, Ruth
dc.contributor.authorCollazos Pulido, Daniela Alejandra
dc.date.accessioned2021-05-28T14:00:28Z
dc.date.available2021-05-28T14:00:28Z
dc.date.issued2020
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/111
dc.description.abstractCryptosporidium spp hace parte de la familia Cryptosporidiidae y del orden Eucoccidiorida. G. duodenalis hace parte de la familia Hexamitidae y orden Diplomonadida. Son protozoarios con amplia distribución a nivel mundial y se encuentran relacionados con problemas digestivos en el ganado vacuno, pequeños rumiantes, animales domésticos e incluso el humano. Los afectados en mayor medida son los animales inmunosuprimidos, especialmente, los animales jóvenes de días o meses de edad pueden cursar síndrome diarreico neonatal, ocasionando importantes pérdidas económicas. Por medio de este estudio descriptivo de corte transversal cuyo objetivo fue detectar la presencia de Cryptosporidium spp y Giardia duodenalis en pequeños rumiantes por medio de IFD en un total de 97 ovinos- caprinos de cuatro veredas de distintos municipios situados en altitudes entre los 1300 y 2500 m.s.n.m. Los datos se obtuvieron por medio de un registro epidemiológico de fincas. La descripción de las variables fue mediante análisis univariados y bivariados con pruebas estadísticas de chi cuadrado y OR, con los posibles factores de riesgo asociados a la presencia de Cryptosporidium y Giardia. La presencia de ooquistes-quistes en cabras fue del 27,2% y en ovejas el 9,33%. Respecto, a la presencia de quistes en cabras fue del 36,3% y en ovejas del 17,3%. Los factores de riesgo asociados fueron; el clima, tipo de explotación intensivo, la limpieza y el manejo de desecho de excretas (abono). Este estudio permitió evidenciar la prevalencia de Cryptosporidium y Giardia en ovinos y caprinos de las cuatros veredas muestreadas, contribuyendo así, al conocimiento de la situación epidemiológica de los protozoarios en cuestión.spa
dc.description.tableofcontentsRESUMEN 1 1. INTRODUCCIÓN 2 2. ANTECEDENTES 4 2.1 Cryptosporidium spp 4 2.2 Giardia duodenalis 11 3. MARCO TEORICO 15 3.1 Generalidades de los ovinos y caprinos 15 3.2 Enfermedades parasitarias más frecuentes 16 3.3 Descripción Cryptosporidium spp en ovejas y cabras 18 3.3.1 Taxonomía 18 3.3.2 Morfología 18 3.3.3 Ciclo de vida 19 3.3.4 Patogenicidad 20 3.3.5 Factores de riesgo 21 3.3.6 Epidemiología 22 3.3.7 Control 24 3.4 Giardia duodenalis en ovejas y cabras 24 3.4.1 Taxonomía 24 3.4.2 Morfología 25 3.4.3 Ciclo de vida 25 3.4.4 Patogenicidad 26 3.4.5 Factores de riesgo 28 3.4.6 Epidemiología 29 3.4.7 Control 30 3.5. Diagnóstico de Cryptosporidium spp y G. duodenalis 30 3.5.1 Inmunofluorescencia Directa 31 4. OBJETIVOS 33 4.1 Objetivo General 33 4.2 Objetivos específicos 33 5. METODOLOGÍA 34 5.1 Tipo de investigación 34 5.2 Población 34 5.3 Tamaño de la Muestra 34 5.4 Recolección de la información 34 5.5 Toma y recolección de las muestras 35 5.6 Procesamiento de las muestras 35 5.6.1 Flotación de ooquistes-quistes 35 5.7 Pruebas diagnosticas 35 5.8 Programas utilizados 37 5.9 Hipótesis 37 5.10 Variables 37 5.10.1 Variable dependiente 37 5.10.2 Variable independiente 37 5.11 Criterios de exclusión 38 5.12 Criterios de inclusión 38 5.13 Técnica de análisis 38 6. RESULTADOS 39 7. DISCUSIÓN 50 8. CONCLUSIONES 55 9. RECOMENDACIONES 56 10. REFERENCIAS 57 ANEXOS 66spa
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dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.rightsDerechos reservados - Universidad Colegio Mayor de Cundinamarca, 2020spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleIdentificación de Cryptosporidium spp y Giardia duodenalis a través de Inmunofluorescencia directa (IFD) en pequeños rumiantes y aspectos epidemiológicos en granjas entre los 1300 y 2500 m.s.n.mspa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotá D.Cspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.lembRumiante
dc.subject.lembEpidemiología
dc.subject.lembParásito
dc.subject.proposalCryptosporidiumspa
dc.subject.proposalGiardiaspa
dc.subject.proposalInmunofluorescencia directa (IFD)spa
dc.subject.proposalfactores de riesgospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.rights.coarhttp://purl.org/coar/access_right/c_14cbspa


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