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Estudio de pseudomonas extremaustralis CMPUJ U515, Bacillus Subtilis ATCC 6633 y Bacillus Thuringiensis Kurstaki HD-1 en relación a estrés: Xenobióticos y Glifosfato

dc.contributor.advisorLópez Pazos, Silvio Alejandro
dc.contributor.advisorEstupiñán Torres, Sandra Mónica
dc.contributor.authorBeltrán Duque, María Angie
dc.date.accessioned2021-06-02T20:00:46Z
dc.date.available2021-06-02T20:00:46Z
dc.date.issued2020-05
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/147
dc.description.abstractLos xenobióticos son compuestos químicos no son naturales como fertilizantes, pesticidas, tintes, dioxinas, bifenilos policlorados o hidrocarburos poliaromáticos; contaminantes, afectan a los seres vivos porque no hacen parte de rutas metabólicas celulares, y son recalcitrantes. En la agricultura se usan xenobióticos como aldrin, dieldrina, endrina, Clordano, Heptacloro-Hexaclorobenceno, glifosato y DDT. Algunos microorganismos capaces de degradar xenobióticos son especies de Pseudomonas y Bacillus con potencial para biodegradación y biorremediación. P. extremaustralis, B. subtilis y B. thuringiensis poseen enzimas de uso biotecnológico. Hasta el 50% de las proteínas de su genoma se consideran hipotéticas de función desconocida, que pueden desempeñar funciones en procesos de adaptación. Este trabajo tiene como objetivo reconocer la posible presencia de proteínas de P. extremaustralis CMPUJ U515, B. subtilis ATCC6633 y B. thuringiensis kurstaki HD-1 que se expresan en condiciones de estrés, incluyendo degradación de xenobióticos. Haciendo uso de tBLASTn (E < 8e-17/ identidad >40% /cobertura >83%) se determinaron enzimas relacionadas con degradación de compuestos xenobióticos, igual que las enzimas glifosato oxidorreductasa o glicina oxidasa involucradas en la vía AMPA y la enzima fosfatasa específica C-P liasa las cuales están asociadas a la capacidad de tomar glifosato como única fuente de carbono o fosforo respectivamente. Las cepas se sometieron a estrés mediado por antibióticos para determinar la posible síntesis de proteínas de choque térmico GroEL/ES identificándose la posible síntesis de GroES (10 kDa). Este trabajo permite comprender mejor mecanismos por los cuales las bacterias se adaptan a ambientes de estrés y permiten identificar su potencial para Biotecnología ambiental.spa
dc.description.tableofcontents1. Título 11 Resumen 11 Introducción12 Objetivos 14 Marco teórico 15 Antecedentes 15 5.3 Pseudomonas extremaustralis 24 5.4 Bacillus subtilis 25 5.5 Bacillus thuringiensis 25 5.6 Proteínas hipotéticas 25 5.7 Xenobióticos en la agricultura 26 5.7.1 Características generales del glifosato 27 5.7.1.1 Propiedades fisicoquímicas del glifosato 27 5.7.1.2 Mecanismos de acción 28 5.8 Biorremediación por Pseudomonas extremaustralis, Bacillus subtilis y Bacillus thuringiensis 29 5.9 Proteínas de shock térmico 30 Metodología 32 Tipo de investigación 32 Universo, población y muestra 32 Universo 32 Población 32 Muestra 32 6.3. Variables 32 Variables independientes 32 Variables dependientes 32 6.4. Hipótesis 33 Hipótesis nula 33 Hipótesis alterna 33 6.5. Indicadores 33 6.6. Técnicas y procedimientos 33 7 Resultados 36 8. Discusión 44 9 Conclusiones 56 10. Bibliografía 58spa
dc.format.extent92p.spa
dc.format.mimetypeapplication/pdfspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleEstudio de pseudomonas extremaustralis CMPUJ U515, Bacillus Subtilis ATCC 6633 y Bacillus Thuringiensis Kurstaki HD-1 en relación a estrés: Xenobióticos y Glifosfatospa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotá D.C.spa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.lembBioaculación
dc.subject.lembFertilizantes
dc.subject.lembPesticidas
dc.subject.proposalPseudomonas extremaustralisspa
dc.subject.proposalBacillus subtilis y Bacillus thuringiensisspa
dc.subject.proposalXenobióticospa
dc.subject.proposalProteína hipotéticaspa
dc.subject.proposalProteína de choque térmicospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
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