dc.contributor.advisor | Vargas Diaz, Luis Eduardo | |
dc.contributor.author | Hernandez Gonzalez, Maritza | |
dc.contributor.author | Herrera Gomez, Yury Katherine | |
dc.date.accessioned | 2021-10-27T20:55:22Z | |
dc.date.available | 2021-10-27T20:55:22Z | |
dc.date.issued | 2018-11 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/3569 | |
dc.description.abstract | Desde que el virus sincitial respiratorio humano (VSRh) fue descubierto, no ha sido posible el desarrollo de profilaxis asequibles que permitan el control de la infección y que a su vez disminuyan los efectos sanitarios y el impacto económico generado por el mismo. El desarrollo de la respuesta inmune adaptativa es uno de los campos que se ha venido explorando como alternativa a dicha problemática.
Es así que, la identificación de péptidos antigénicos capaces de formar complejos MHC clase II HLA-DR/péptido resulta de gran importancia como base para la búsqueda de una vacuna segura y eficaz. En este trabajo de investigación se evaluó la capacidad de unión de ocho péptidos provenientes de las proteínas F, L, N, M, G y SH del VSRh para formar complejo HLA-DR/péptido con los alelos HLA- DR1 Y HLA-DR4 del complejo mayor de histocompatibilidad clase II. Para ello se empleó el algoritmo PROPRED y un ensayo de unión competitiva en presencia y ausencia de HLA-DM.
Los resultados demuestran una correlación del 68% entre los datos teóricos y experimentales en ausencia de HLA-DM. Por otro lado, se observa que N-298 y L-962 son los péptidos de mayor afinidad por los alelos HLA-DR1 y HLA-DR4, indicando que pueden ser inmunógenos capaces de generar una respuesta inmunitaria efectiva contra el VSRh en individuos HLA-DR1 o HLA-DR4. De manera similar, los resultados sugieren que los péptidos SH-50, G1-58 Y L-832 pueden tener un papel similar a HLA-DM en la modulación de la formación del complejo MHC clase II/péptido. | spa |
dc.description.tableofcontents | RESUMEN12
INTRODUCCIÓN13
1. OBJETIVOS15
1.1 OBJETIVO GENERAL15
1.2 OBJETIVOS ESPECÍFICOS15
2. JUSTIFICACIÓN16
3. ANTECEDENTES18
4. MARCO TEÓRICO23
4.1 Moléculas MHC clase II: Estructura, función y características de la unión HLA/ péptido23
4.2 Virus sincitial respiratorio humano28
4.2.1 Proteínas no estructurales31
4.2.2 Proteína N31
4.2.3 Proteína M32
4.2.4 Proteína F33
4.2.5 Proteína SH (Small Hydrophobic36
4.2.6 Proteína G38
4.2.7 Proteína L40
4.2.8 Proteína P42
4.3 Respuesta inmune frente al Virus Sincitial Respiratorio Humano43
4.4 Manifestaciones clínicas y diagnóstico del VSRh44
4.5 Epidemiologia44
4.6 Tratamiento y protección frente al VSRh46
4.7 Ensayos inmunoenzimáticos (ELISA48
4.8 Algoritmo predictivo RAGHAVA-PROPRED48
5. DISEÑO METODOLÓGICO50
5.1 Moléculas MHC clase II y HLA-DM50
5.2 Péptido control biotinilado: Hemaglutinina (HA) 306-31850
5.3 Péptidos del virus sincitial respiratorio51
5.4 Determinación del porcentaje de unión empleando el algoritmo PROPRED53 7
5.5 Ensayo de unión MHC clase II/ Péptidos54
5.6 Ensayo de Unión MHC clase II/ Péptidos / HLA-DM56
5.7 Determinación del porcentaje de unión relativo MHC clase II/péptido56
6. RESULTADOS57
6.1 Determinación del porcentaje de unión de los péptidos F-137, L-962, N-298, L-832, L-449, M-68, G1-58 a los alelos HLA-DRB1* 0101 y HLA-DRB1*0401 empleando el algoritmo PROPRED57
6.2 Determinación experimental de los porcentajes de unión relativos de péptidos del VSRh a moléculas HLA-DR1 Y HLA-DR4 en presencia y ausencia de HLA-DM59
6.3 Comparación del porcentaje de unión de cada péptido con HLA-DR1 y HLA- DR4 en presencia y ausencia de HLA-DM63
7. DISCUSIÓN67
8. CONCLUSIONES73
RECOMENDACIONES76
REFERENCIAS77
ANEXOS90 | spa |
dc.format.extent | 95p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.publisher | Universidad Colegio Mayor de Cundinamarca | spa |
dc.rights | Universidad Colegio Mayor de Cundinamarca, 2019 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Evaluación de la unión de ocho péptidos del virus Sincitial respiratorio humano a los alelos MHC clase ii: HLA -DR1 Y HLA - DR4. | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | spa |
dc.identifier.barcode | 58651 | |
dc.publisher.faculty | Facultad de Ciencias de la Salud | spa |
dc.publisher.place | Bogotá D.C | spa |
dc.publisher.program | Bacteriología y Laboratorio Clínico | spa |
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dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.lemb | El Virus Sincitial Respiratorio | |
dc.subject.lemb | Patologías Neuromusculares | |
dc.subject.lemb | Bronquiolitis | |
dc.subject.lemb | Paramyxoviridae | |
dc.subject.proposal | Presentación de antígenos | spa |
dc.subject.proposal | Selección de Epítopes | spa |
dc.subject.proposal | Complejo MHC II/Péptido | spa |
dc.subject.proposal | Susceptibilidad HLA-DM | spa |
dc.subject.proposal | Algoritmos de unión a moléculas MHC clase II | spa |
dc.subject.proposal | Moléculas HLA-DR1 y DR4 | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |