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Efecto de la salinidad sobre genes asociados a ciclos biogeoquímicos del manglar de la desembocadura del Río Ranchería, La Guajira.

dc.contributor.advisorVanegas Guerrero, Javier
dc.contributor.advisorPosada Buitrago, Martha Lucía
dc.contributor.authorSandoval Figueredo, Angie Vanessa
dc.contributor.authorRodelo Bernal, María Camila
dc.date.accessioned2021-11-11T14:49:14Z
dc.date.available2021-11-11T14:49:14Z
dc.date.issued2019
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/3643
dc.description.abstractLos suelos de manglar albergan una gran variedad de microorganismos claves en el ciclaje de nutrientes que actúan bajo variaciones estacionales y diarias de salinidad. Sin embargo, se desconoce cómo la salinidad influye sobre la actividad funcional de los microorganismos del manglar. El objetivo de este trabajo fue determinar el efecto de la salinidad sobre genes asociados a ciclos biogeoquímicos Nitrógeno, Azufre y Metano en un manglar alterado de la Guajira por medio de un análisis metagenómico. Para esto se muestrearon tres puntos contrastantes en salinidad (H: 61,52 ‰, M: 14,61 ‰, L: 2,80 ‰), se extrajo el ADN total, se secuenció por Illumina HiSeq, se anotó con MEGAN 5 y se asignaron las secuencias con la base de datos KEGG. El análisis estadístico se realizó con la página web Microbiome Analyst y STAMP. Se encontró que los genes asociados al ciclo del Metano tuvieron las mayores abundancias, seguido de los genes del ciclo del N y S. El ciclo del Metano fue favorecido por la salinidad. Se detectaron más marcadores en salinidad alta entre los tres ciclos. Sin embargo, la salinidad baja favoreció la abundancia de glutamina sisntetasa (glnA) del ciclo del N, la salinidad media de sulfato adeniltransferasa (sat) del ciclo del S y la salinidad alta de la piruvato, agua dikinasa (pps) del ciclo del Metano. Los resultados revelan la influencia de salinidad sobre los ciclos biogeoquímicos y contribuyen a entender la dinámica funcional de los microorganismos del manglar.spa
dc.description.abstractMangrove soils harbor a great variety of microorganisms that are key in the cycling of nutrients that act under seasonal and daily salinity variations. However, it is unknown how salinity influences the functional activity of mangrove microorganisms. The objective of this work was determine the effect of salinity on genes associated to Nitrogen, Sulfur and Methane biogeochemical cycles in an altered mangrove swamp of Guajira by metagenomics. For this, three contrasting salinity points were sampled (H: 61.52 ‰, M: 14.61 ‰, L: 2.80 ‰), the total DNA was extracted, sequenced by illumina HiSeq, metagenome annotation with MEGAN 5 and the sequences were assigned with the KEGG database. The statistical analysis was carried out with the Microbiome Analyst website and STAMP. It was found that genes associated with methane cycle had the highest abundances, followed by the N and S cycle genes. The methane cycle was favored by salinity. In high salinity, more biomarkers were found among the three cycles. However, these indicated that the low salinity favors the abundance of glutamine synthetase (glnA) of the N cycle; in medium salinity the sulfate adenyltranferase (sat) of the S cycle and in high salinity, the pyruvate water dikinase (pps) of Methane cycle. The results reveal the influence of salinity on biogeochemical cycles and contribute to understand the functional dynamics of mangrove microorganisms.eng
dc.description.tableofcontentsAbreviaturas 11 Resumen 1 1. 16 2. 18 2.1 Objetivo general 5 2.2 Objetivos específicos 5 3. 19 3.1 Manglares en Colombia 6 3.2 Metagenómica en Colombia 6 3.3 Secuenciación masiva en Manglares 6 3.5 Efecto de la salinidad sobre microorganismos de manglar 9 4. 23 4.1 23 4.1.1 23 4.2 24 4.2.1 Papel de los microorganismos en los ciclos biogeoquímicos 11 4.2.2 Ciclo del Nitrógeno 11 4.2.3 Ciclo del Azufre 13 4.2.4 Ciclo del Carbono 14 4.3 Metagenómica 15 4.3.1 Definición y características 15 4.3.2 Herramientas bioinformáticas de análisis metagenómico 16 4.3.2.1 Megan 5.0 16 4.3.2.2 KEGG 16 4.3.2.3 Microbiome analyst 17 4.3.2.3.1 Lefse 17 4.3.2.3.2 Biomarcador 17 4.3.2.3.3 Metagenomeseq 17 4.3.2.4 STAMP 18 4.3.2.4.1 Análisis de componentes principales PCA 18 4.3.2.4.2 Mapa de calor 18 5. 32 5.1 Universo, población, muestra 19 5.2 Hipótesis, variables e indicadores 19 5.3 Técnicas y procedimientos 20 3. 36 6.1 Ciclo del Metano 24 6.2 Ciclo del Nitrógeno 27 6.3 Ciclo del Azufre 29 6.4 Biomarcadores 31 4. 48 5. 52 Referencias Bibliográficas 40 Anexos 57spa
dc.format.extent83p.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.rightsDerechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2019spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleEfecto de la salinidad sobre genes asociados a ciclos biogeoquímicos del manglar de la desembocadura del Río Ranchería, La Guajira.spa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.identifier.barcode60032
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotá DCspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.subject.lembazufre
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dc.subject.proposalSalinidadspa
dc.subject.proposalManglarspa
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