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Frecuencia Alélica Y Genotipica De Hla-B Y Hla-B*27 En Un Grupo De Individuos Sanos En Colombia

dc.contributor.advisorRomero Sánchez, María Consuelo
dc.contributor.advisorChila Moreno, Leidy Lorena
dc.contributor.advisorInfante Cruz, Alejandra Del Pilar
dc.contributor.authorHernández Bocanegra, Natalia Andrea
dc.date.accessioned2021-11-22T01:48:22Z
dc.date.available2021-11-22T01:48:22Z
dc.date.issued2019-06
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/3698
dc.description.abstractVarios de los genes de la respuesta inmune se encuentran en el complejo mayor de histocompatibilidad (MHC). El sistema de antígenos de leucocitos humanos (HLA) es un conjunto de moléculas de superficie de células altamente polimórficas que se unen a péptidos derivados del procesamiento antigénico con la finalidad de presentárselos a los linfocitos T, para inducir su activación y función efectora. Dentro de los alelos de clase I se encuentran el HLA-B y su subtipo HLA-B*27 que está altamente asociado con la presencia de enfermedades musculo esqueléticas. El objetivo de este proyecto de investigación es la caracterización de los genotipos y alelos HLA-B y su subtipo B*27 específicamente en una población de individuos sanos colombianos y así evidenciar la variabilidad genética producto del mestizaje. La muestra comprendió 255 individuos sanos, sin clínica sugestiva de enfermedades reumáticas, remitidos al laboratorio de Inmunología del Hospital Militar Central desde el año 2015 al 2018 con solicitud de tipificación HLA- A, B, DR por sistema molecular SSO- LUMINEX IS 100/200. Los resultados mostraron 27 genotipos y 89 alelos HLA-B, así mismo, trece sujetos fueron positivos para el antígeno B*27 correspondientes a los alelos más frecuentemente reportados en población colombiana y latinoamericana.spa
dc.description.tableofcontentsINTRODUCCIÓN 1 1. OBJETIVOS 3 1.1. OBJETIVO GENERAL 4 1.2. OBJETIVOS ESPECÍFICOS 4 2. ANTECEDENTES 4 3. MARCO TEORICO 11 3.1. COMPLEJO MAYOR DE HISTOCOMPATIBILIDAD 11 3.2. ANTIGENO LEUCOCITARIO HUMANO HLA 12 3.3. MOLECULAS HLA 14 3.4. PROCESAMIENTO DE ANTIGENOS 16 3.4.1. Procesamiento péptidos clase I 16 3.4.2. Procesamiento de péptidos por HLA-II 21 3.5. HLA-B*27 Y ASOCIACIÓN CON ESPONDILITIS ANQUILOSANTE 24 4. DISEÑO METODOLÓGICO 26 4.1. UNIVERSO, POBLACIÓN, MUESTRA 26 4.1.1. Tipo de investigación 26 4.1.2. Población 26 4.1.3. Muestra 26 4.1.4. Selección de la muestra 26 4.1.5. Criterios de inclusión 27 4.1.6. Criterios de Exclusión 27 4.1.7. Análisis de tipificación de antígeno HLA-B 27 4.1.8. Análisis estadístico 28 4.2. HIPÓTESIS 28 4.3. TÉCNICAS Y PROCEDIMIENTOS 28 4.3.1. Extracción de DNA 28 4.3.2. Cuantificación 30 4.3.3. Amplificación por PCR 31 4.3.4. Hibridación 32 4.3.5. Tipificación HLA-B27 por técnica de Luminex IS 100/200 33 4.3.6. Secuenciación HLA de alta resolución 33 5. RESULTADOS 34 5.1. Descripción de la muestra 34 5.2. Frecuencia Genotípica del HLA -B 36 5.3. Frecuencia alélica del HLA-B 38 5.4. Frecuencia Haplotípica 41 5.5. Frecuencia HLA-B*27 42 6. DISCUSIÓN 44 7. CONCLUSIONES 51 8. REFERENCIAS 52 9. ANEXOS 63 9.1 Resultados secuenciación de alta resolución 63spa
dc.format.extent75p.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.rightsDerechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2019spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleFrecuencia Alélica Y Genotipica De Hla-B Y Hla-B*27 En Un Grupo De Individuos Sanos En Colombiaspa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.identifier.barcode60085
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotá DCspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.accessrightsinfo:eu-repo/semantics/closedAccessspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.lembfrecuencia genotípica
dc.subject.lembsistema LUMINEX IS 100/200
dc.subject.proposalHLA-Bspa
dc.subject.proposalHLA-B*27spa
dc.subject.proposalfrecuencia alélicaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.rights.coarhttp://purl.org/coar/access_right/c_14cbspa


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