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Identificación de polimorfismos en el gen Mieloperoxidasa y su papel en Candidiasis Vulvovaginal recurrente por Candida SPP en pacientes de Bogotá DC

dc.contributor.advisorCruz Baquero, Claudia Andrea
dc.contributor.advisorPinilla Bermúdez, Gladys
dc.contributor.authorMontoya Estrada, María Paula
dc.contributor.authorRincón González, Rincón González
dc.date.accessioned2022-10-18T17:27:13Z
dc.date.available2022-10-18T17:27:13Z
dc.date.issued2022-04-08
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/5717
dc.description.abstractLa mieloperoxidasa (MPO) es una proteína antimicrobiana presente en los gránulos azurófilos de los neutrófilos, su acción recae cuando se conjuga en el fagosoma con peróxido de hidrógeno, resultando en sustancias tóxicas para los microorganismos fagocitados (1). Se ha descrito que las infecciones vulvovaginales recurrentes por Candida spp tienen relación con la deficiencia priMaría de MPO, el cual es un trastorno autosómico recesivo (2), que presenta polimorfismos asociados al procesamiento postraduccional defectuoso de la proteína precursora o con errores pretraduccionales (3), por esto, el propósito fue identificar la relación que existe entre dos polimorfismos de la MPO (Y173C y M251T) con una mayor probabilidad de presentar este tipo de infecciones, puesto que este tipo de polimorfismos no han sido estudiados en el país. Frente a la metodología, se realizó una encuesta, donde se recolectaron 27 muestras de mujeres entre 20-45 años, se les realizó cuadro hemático, tinción Wright y tinción de peroxidasa leucocitaria. Se prosiguió con la extracción de ADN utilizando el kit Wizard® Genomic DNA Purification de Promega, luego se realizó la cuantificación del ADN extraído por espectrofotometría en NanoDrop, y la evaluación de la integridad del ADN mediante electroforesis. Por otro lado, se diseñaron los primers haciendo uso de herramientas bioinformáticas para la estandarización de la PCR convencional, posteriormente se usaron enzimas de restricción y se secuenciaron los productos amplificados con el fin de identificar los polimorfismos mencionados previamente.spa
dc.description.tableofcontentsTABLA DE CONTENIDO RESUMEN. 12 INTRODUCCIÓN . 13 1. ANTECEDENTES DEL PROYECTO .. 17 2. MARCO REFERENCIAL... 18 2.1 Sistema inmune . 18 2.2 Neutrófilos.. 19 2.3 Mieloperoxidasa 20 2.4 Polimorfismos relacionados con la deficiencia de MPO. 21 2.5 Inmunodeficiencias... 22 2.6 Candida spp 22 2.7 Patogenia de Candida spp... 23 2.8 Generalidades de candidiasis.. 23 2.9 Epidemiología 24 2.10 Diagnóstico.. 24 2.11 Tratamiento 25 2.12 Deficiencia de Mieloperoxidasa y susceptibilidad a infecciones por Candida spp. 25 2.13 Diagnóstico deficiencia de MPO. 26 2.13.1 Técnicas citoquímicas.. 26 2.13.2 PCR convencional .. 27 2.13.3 Secuenciación de ADN. 27 3. DISEÑO METODOLÓGICO. 27 3.1 Universo, Población y Muestra... 27 3.2 Hipótesis y Variables... 28 3.3. Técnicas y procedimientos 28 3.3.1 Recolección de muestras de sangre 28 3.3.2 Consentimiento informado .. 28 3.3.3 Análisis parámetros hemáticos ... 28 3.3.4 Identificación de peroxidasa leucocitaria 29 3.3.5 Identificación del gen mpo 29 3.3.6 Enzimas de restricción y electroforesis en gel de agarosa .. 30 3.3.7 Detección de polimorfismo del gen mpo... 30 4. RESULTADOS 31 4.1 Análisis de parámetros hemáticos 31 4.2 Identificación del gen mpo. 31 4.3 Enzimas de restricción y electroforesis en gel de agarosa. 33 4.4 Detección de polimorfismos del gen mpo.. 33 4 DISCUSIÓN. 34 5 CONCLUSIONES.. 39 REFERENCIAS... 41spa
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dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.rightsDerechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleIdentificación de polimorfismos en el gen Mieloperoxidasa y su papel en Candidiasis Vulvovaginal recurrente por Candida SPP en pacientes de Bogotá DCspa
dc.typeTrabajo de grado - Pregradospa
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotáspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.proposalPolimorfismosspa
dc.subject.proposalDeficiencia de Mieloperoxidasaspa
dc.subject.proposalAnálisis Bioinformáticospa
dc.subject.proposalPCRspa
dc.subject.proposalSecuenciaciónspa
dc.subject.proposalCandidiasis vulvovaginalspa
dc.subject.proposalCandida sppspa
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dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
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