dc.contributor.advisor | Cruz Baquero, Claudia Andrea | |
dc.contributor.advisor | Pinilla Bermúdez, Gladys | |
dc.contributor.author | Montoya Estrada, María Paula | |
dc.contributor.author | Rincón González, Rincón González | |
dc.date.accessioned | 2022-10-18T17:27:13Z | |
dc.date.available | 2022-10-18T17:27:13Z | |
dc.date.issued | 2022-04-08 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5717 | |
dc.description.abstract | La mieloperoxidasa (MPO) es una proteína antimicrobiana presente en los gránulos azurófilos de los
neutrófilos, su acción recae cuando se conjuga en el fagosoma con peróxido de hidrógeno, resultando
en sustancias tóxicas para los microorganismos fagocitados (1). Se ha descrito que las infecciones
vulvovaginales recurrentes por Candida spp tienen relación con la deficiencia priMaría de MPO, el
cual es un trastorno autosómico recesivo (2), que presenta polimorfismos asociados al
procesamiento postraduccional defectuoso de la proteína precursora o con errores pretraduccionales
(3), por esto, el propósito fue identificar la relación que existe entre dos polimorfismos de la MPO
(Y173C y M251T) con una mayor probabilidad de presentar este tipo de infecciones, puesto que este
tipo de polimorfismos no han sido estudiados en el país. Frente a la metodología, se realizó una
encuesta, donde se recolectaron 27 muestras de mujeres entre 20-45 años, se les realizó cuadro
hemático, tinción Wright y tinción de peroxidasa leucocitaria. Se prosiguió con la extracción de
ADN utilizando el kit Wizard® Genomic DNA Purification de Promega, luego se realizó la
cuantificación del ADN extraído por espectrofotometría en NanoDrop, y la evaluación de la
integridad del ADN mediante electroforesis. Por otro lado, se diseñaron los primers haciendo uso de
herramientas bioinformáticas para la estandarización de la PCR convencional, posteriormente se
usaron enzimas de restricción y se secuenciaron los productos amplificados con el fin de identificar
los polimorfismos mencionados previamente. | spa |
dc.description.tableofcontents | TABLA DE CONTENIDO
RESUMEN. 12
INTRODUCCIÓN . 13
1. ANTECEDENTES DEL PROYECTO .. 17
2. MARCO REFERENCIAL... 18
2.1 Sistema inmune . 18
2.2 Neutrófilos.. 19
2.3 Mieloperoxidasa 20
2.4 Polimorfismos relacionados con la deficiencia de MPO. 21
2.5 Inmunodeficiencias... 22
2.6 Candida spp 22
2.7 Patogenia de Candida spp... 23
2.8 Generalidades de candidiasis.. 23
2.9 Epidemiología 24
2.10 Diagnóstico.. 24
2.11 Tratamiento 25
2.12 Deficiencia de Mieloperoxidasa y susceptibilidad a infecciones por Candida spp. 25
2.13 Diagnóstico deficiencia de MPO. 26
2.13.1 Técnicas citoquímicas.. 26
2.13.2 PCR convencional .. 27
2.13.3 Secuenciación de ADN. 27
3. DISEÑO METODOLÓGICO. 27
3.1 Universo, Población y Muestra... 27
3.2 Hipótesis y Variables... 28
3.3. Técnicas y procedimientos 28
3.3.1 Recolección de muestras de sangre 28
3.3.2 Consentimiento informado .. 28
3.3.3 Análisis parámetros hemáticos ... 28
3.3.4 Identificación de peroxidasa leucocitaria 29
3.3.5 Identificación del gen mpo 29
3.3.6 Enzimas de restricción y electroforesis en gel de agarosa .. 30
3.3.7 Detección de polimorfismo del gen mpo... 30
4. RESULTADOS 31
4.1 Análisis de parámetros hemáticos 31
4.2 Identificación del gen mpo. 31
4.3 Enzimas de restricción y electroforesis en gel de agarosa. 33
4.4 Detección de polimorfismos del gen mpo.. 33
4 DISCUSIÓN. 34
5 CONCLUSIONES.. 39
REFERENCIAS... 41 | spa |
dc.format.extent | 83p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.rights | Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Identificación de polimorfismos en el gen Mieloperoxidasa y su papel en Candidiasis Vulvovaginal recurrente por Candida SPP en pacientes de Bogotá DC | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | spa |
dc.publisher.faculty | Facultad de Ciencias de la Salud | spa |
dc.publisher.place | Bogotá | spa |
dc.publisher.program | Bacteriología y Laboratorio Clínico | spa |
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dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.proposal | Polimorfismos | spa |
dc.subject.proposal | Deficiencia de Mieloperoxidasa | spa |
dc.subject.proposal | Análisis Bioinformático | spa |
dc.subject.proposal | PCR | spa |
dc.subject.proposal | Secuenciación | spa |
dc.subject.proposal | Candidiasis vulvovaginal | spa |
dc.subject.proposal | Candida spp | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |