dc.contributor.advisor | López Pazos, Silvio Alejandro | |
dc.contributor.advisor | Estupiñan Torres, Sandra Mónica | |
dc.contributor.author | Sánchez Murcia, Paola Andrea | |
dc.date.accessioned | 2022-05-11T02:36:36Z | |
dc.date.available | 2022-05-11T02:36:36Z | |
dc.date.issued | 2021-09 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5555 | |
dc.description.abstract | Desde hace 21 años se han venido estudiando unas proteínas producidas por Bacillus
thuringiensis en su fase de esporulación, denominadas parasporinas (PS), las cuales han
demostrado tener un efecto citotóxico sobre las células cancerígenas humanas de diversos
orígenes, caracterizándose por poseer propiedades entomopatógenas, no son hemolíticas y por
presentan una baja o nula citotoxicidad hacia las células sanas. En este trabajo se analizó la
diversidad de las PS en genomas procariotas, determinando en ellos sus propiedades
bioquímicas, por otra parte se identificaron patentes relacionadas con PS. Las secuencias se
analizaron por medio de las herramientas bioinformáticas BLAST-P, ProtParam y la base de
datos para patentes THE LENS. Se manejaron 78 genomas de bacterias y 12 de arqueas, los
cuales fueron comparados con las secuencias de aminoácidos de las 19 PS experimentalmente
determinadas, obteniendo 1404 y 228 proteínas similares a las PS, respectivamente, estas
secuencias se filtraron con base en parámetros de cobertura ≥ 60%, el valor E más cercano a 0 y
un porcentaje de identidad ≥20%, obteniendo finalmente 16 proteínas de bacterias y 3 de
arqueas, encontrando que tienen actividad intracelular, son proteínas estables, hidrófilas y
resistentes a cambios repentinos en su estructura física o química. A partir de la búsqueda de
patentes se identificó la existencia de dos patentes activas relacionadas con PS. Con este estudio
se pudo determinar que los géneros Bacillus, Vibrio, Clostridium y Nanoarchaeum producen
proteínas tipo PS y además que a la fecha se cuenta con dos patentes activas asociadas a las
mismas. | spa |
dc.description.tableofcontents | RESUMEN 10
Introducción 12
Objetivos 14
Objetivo general 14
Objetivos específicos 14
1. Antecedentes 15
2. Marco Referencial 21
2.1. Características de Bacillus thuringiensis 21
2.2. Proteínas Cry 22
2.3. Mecanismo de acción de proteínas Cry y Mpp 24
2.3.1. Modelo de formación de poros (MFP) de proteínas Cry. 24
2.3.2. Modelo de Transducción de señal (MTS) de proteínas Cry. 25
2.3.3. Modelo de formación de poros de proteínas Mpp. 26
2.4. Parasporinas y sus generalidades. 28
2.5. Nomenclatura y estructura de parasporina 29
2.6. Patentes 30
2.6.1. Base de datos de patentes The Lens 31
3. Diseño metodológico 33
3.1. Universo, población y muestra 33
3.2. Hipótesis, variables, indicadores 33
3.3. Técnicas y procedimientos 33
3.3.1 Secuencias de parasporina. 33
3.3.2. Análisis con BLAST-P para la identificación de secuencias similares a PS en
genomas de bacterias y arqueas 33
3.3.3. Análisis bioquímico de las secuencias de aminoácidos tipo PS. 34
3.3.4. Patentes. 34
4. Resultados 35
4.3. Identificación entre secuencias de PS con géneros de bacterias y arqueas 35
4.4. Caracterización bioquímica de las proteínas 36
4.5. Patentes 40
5. Discusión 45
5.3. Alineamiento entre secuencias de PS con géneros de bacterias y arqueas 45
5.4. Caracterización bioquímica de las proteínas 46
5.5. Patentes 48
6. Conclusiones. 49
Referencias bibliográficas 50 | spa |
dc.format.extent | 60p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.publisher | Universidad Colegio Mayor de Cundinamarca | spa |
dc.rights | Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2021 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Identificación de la diversidad de parasporinas de Bacillus thuringiensis en organismos procariotas | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | spa |
dc.publisher.faculty | Facultad de Ciencias de la Salud | spa |
dc.publisher.place | Bogotá | spa |
dc.publisher.program | Bacteriología y Laboratorio Clínico | spa |
dc.relation.references | Portela D, Chaparro A, Lopez S. La biotecnología de Bacillus thuringiensis. NOVA
[Internet]. 2013 [Citado 17 feb 2021], Vol. 11, (20), págs. 87-96. Disponible en:
http://www.scielo.org.co/pdf/nova/v11n20/v11n20a09.pdf. | spa |
dc.relation.references | Fang Y, Li Z, Liu J, Shu C, Wang X, Zhang X, et al. A pangenomic study of Bacillus
thuringiensis. J of Genetics and Genomics [Internet]. 2011 [Cited 16 sept 2020], Vol. 38, (12),
pp. 567-576. Available in:
https://www.sciencedirect.com/science/article/abs/pii/S1673852711001895 | spa |
dc.relation.references | Bravo A, Gómez I, Porta H, García-Gómez B, Rodriguez-Almazan C, Pardo L, et al.
Evolution of Bacillus thuringiensis Cry toxins. J Biotechnology & Applied Microbiology
[Internet]. 2012 [Cited 19 sept 2020], Vol. 6, (1), pp. 17-26. Available in:
https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1751-7915.2012.00342.x. | spa |
dc.relation.references | Mizuki E, Park Y, Saitoh H, Yamashita S, Akao T, Higuchi K, et al. Parasporin, a Human
Leukemic Cell-Recognizing Parasporal. J Clinical and Vaccine Immunology [Internet]. 2000
[Cited 30 oct 2020], Vol. 7, (4), pp. 625–634. Available in: https://cvi.asm.org/content/7/4/625. | spa |
dc.relation.references | Okumura S, Koga H, Inouye K, Mizuki E. Toxicity of Parasporin-4 and Health Effects of
Pro-parasporin-4 Diet in Mice. Toxins. J Toxins [Internet]. 2014 [Cited 31 oct 2020], Vol. 6, (7),
pp. 2115-2126. Available in: https://www.mdpi.com/2072-6651/6/7/2115. | spa |
dc.relation.references | National Center for Biotechnology Information (NCBI). Genome. Genome Information by
Organism. [Online] 1988 [Query: 31 oct 2020]. [Cited: Ago 29, 2021.]
https://www.ncbi.nlm.nih.gov/genome/browse#!/prokaryotes/bacillus%20thuringiensis. | spa |
dc.relation.references | Ginebra. WIPO. WIPO Patent Drafting Manual. IP ASSETS MANAGEMENT SERIES. (2017
)[Cited May 23 2021]. págs. 1-138. Available
in:https://www.wipo.int/edocs/pubdocs/en/patents/867/wipo_pub_867.pdf. | spa |
dc.relation.references | Hamm RL, Gregg A, Sparks TC. Intellectual property in entomology: Analysis and
perspective on recent trends in global patent publications. Pest Manag Sci [Internet]. 2020
[Cited 29 Aug 2021], Vol. 76, (5), págs. 1603-1611. Available in:
https://onlinelibrary.wiley.com/doi/10.1002/ps.5780. | spa |
dc.relation.references | Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H, Park YS. Unique activity associated
with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on
human cancer cells. J. Applied Microbiology [Inernet]. 1999[Cited May 23 2020 ], Vol. 86, (3),
págs. 477–486. Available in:
https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1046/j.1365-2672.1999.00692.x. | spa |
dc.relation.references | Wu J, Zhao F, Bai J, Deng G, Qin S, Bao Q. Adaptive Evolution of cry Genes in Bacillus
thuringiensis: Implications for Their Specif icity Determination. J Genomics, Proteomics and
Bioinformatics [Internet]. 2007 [Cited 16 sept 2020], Vol. 5, (2), pp. 102-110. Available in:
https://www.sciencedirect.com/science/article/pii/S1672022907600205?via%3Dihub#bibliog000
5. | spa |
dc.relation.references | Nagamatsu Y, Okamura S, Saitou H, Akao T, Mizuki E. Three Cry toxins in two types
from Bacillus thuringiensis strain M019 preferentially kill human hepatocyte cancer and uterus
cervix cancer cells. Biosci Biotechnol Biochem [Internet]. 2010 [Cited 20 Jul 2020], Vol. 74, (3),
págs. 494–498. Available in: https://academic.oup.com/bbb/article/74/3/494/5949553. | spa |
dc.relation.references | Lucena W, Pelegrini P, Martins-de-Sa D, Fonseca F, Gomes J, de Macedo L, et al.
Molecular Approaches to Improve the Insecticidal Activity of. J Toxins [Internet]. 2014 [Cited
24 sept 2020], Vol. 6, (8), pp. 2393-2423. Available in:
https://www.mdpi.com/2072-6651/6/8/2393/htm. | spa |
dc.relation.references | Xu C, Wang B-C, Yu Z, Sun M. Structural Insights into Bacillus thuringiensis Cry, Cyt and
Parasporin Toxins. J Toxins [Internet]. 2014 [Cited 30 oct 2020], Vol. 6, (9), pp. 2732-2770.
Available in:https://www.mdpi.com/2072-6651/6/9/2732/htm. | spa |
dc.relation.references | Masri L, Branc A, Sheppard AE, Papkou A, Laehnemann D, Guenther PS, et al.
Host–Pathogen Coevolution: The Selective Advantage of Bacillus thuringiensis Virulence and Its
Cry Toxin Genes. J PLOS Biology [Internet]. 2015 [Cited 28 sept 2020], Vol. 13, (6), pp. 1-30.
Available in: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002169 | spa |
dc.relation.references | Pacheco S, Cantón E, Zuñiga-Navarrete F, Pecorari F, Bravo A, Soberón M.
Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and
ribosomes. J AMB Express [Internet]. 2015 [Cited 27 sept 2020], Vol. 5, (73), pp. 1-10.
Available in: https://amb-express.springeropen.com/articles/10.1186/s13568-015-0160-1. | spa |
dc.relation.references | Brasseur K, Auger P, Asselin E, Parent S, Côté JC, Sirois M. Parasporin-2 from a New
Bacillus thuringiensis 4R2 Strain Induces Caspases Activation and Apoptosis in Human Cancer
Cells. PLoS One [Internet]. 2015 [Cited 23 mar 2021], Vol. 10, (8), págs. 1-22. Avalable in:
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00708/full. | spa |
dc.relation.references | Zghal RZ, Elleuch J, Ben-Ali M, Darriet F, Rebaï A, Chandre F, et al. Towards novel Cry
toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin. J Applied
Microbiology and Biotechnology [Internet]. 2016 [Cited 27 sept 2020], Vol. 101, (1), pp.
113-122. Available in: https://link.springer.com/article/10.1007/s00253-016-7766-3. | spa |
dc.relation.references | Reyes J. Obtención de parasporinas de Bacillus thuringiensis cepas CL9-1 CL9-3 Y CL9-21
con actividad citótoxica para células de origen neoplásico [Trabajo de grado Maestria en ciencias
con orientación en microbiologia aplicada]. Mexico: Universidad Autónoma de nuevo León
Facultad de ciencias químicas; 2016. Disponible en:
http://eprints.uanl.mx/14439/1/1080252167.pdf. | spa |
dc.relation.references | Pinzon E, Sierra D, Suarez M, Orduz S, Florez A. DNA secondary structure formation by
DNA shuffling of the conserved domains of the Cry protein of Bacillus thuringiensis. J BMC
Biophysics [Internet]. 2017 [Cited 28sept 2020], Vol. 10, (4), pp. 1-10. Available in:
https://bmcbiophys.biomedcentral.com/articles/10.1186/s13628-017-0036-7#article-info. | spa |
dc.relation.references | Zheng J, Gao Q, Liu L, Liu H, Wang Y, Peng D, et al. Comparative Genomics of Bacillus
thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts. J mBio
[Internet]. 2017 [Cited 29 sept 2020], Vol. 8, (4), pp. 1-14. Available in:
https://mbio.asm.org/content/8/4/e00822-17/article-info. | spa |
dc.relation.references | Fiedoruk K, Daniluk T, Mahillon J, Leszczynska K, Swiecicka I. Genetic Environment of
cry1 Genes Indicates Their Common Origin. J Genome Biology and Evolution [Internet]. 2017
[Cited 2 oct 2020], Vol. 9, (9), pp. 2265-2275. Available in:
https://academic.oup.com/gbe/article/9/9/2265/4091611. | spa |
dc.relation.references | Beena V, Ramnath V, SreekumarK, Karthiayini K, Philomina P, Girija D. Crystal
Protein of a Novel Bacillus thuringiensis Strain Inducing Cell Cycle Arrest and Apoptotic Cell
Death in Human Leukemic Cells. J Scientific Reports [Internet]. 2019 [Cited 04 nov 2020], Vol.
9, (1), pp. 1-9. Available in: https://www.nature.com/articles/s41598-019-45928-z. | spa |
dc.relation.references | Crickmore N, Berry C, Panneerselvam S, MishraR, Connor T, Bonning B. A
structure-based nomenclature for Bacillus thuringiensis and other bacteria-derived pesticidal
proteins [Internet]. J.l of Invertebrate Pathology. 2020 [17 Mar 2021], Vol. 107438, pp. 1-5.
Available in: https://www.sciencedirect.com/science/article/pii/S0022201120301440. | spa |
dc.relation.references | Japón. Kyushu Univ. Parasporin-2 receptor active on cancer, and use thereof. (2009) [Cited
14 May 2021]. Available in:
https://patents.google.com/patent/JP2011068568A/en?oq=JP+2011068568+A. | spa |
dc.relation.references | Nair K, Al-Thani R, Al-Thani D, Al-Yafei F, Ahmed T, Jaoua S. Diversity of Bacillus
thuringiensis Strains From Qatar as Shown by Crystal Morphology, δ-Endotoxins and Cry Gene
Content. Front. Microbiol [Internet]. 2018 [cited 15 Jan 2021], Vol. 9, (708), pp. 1-10. Available
in: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00708/full. | spa |
dc.relation.references | Pigott C, Ellar D. Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity.
Microbiol Mol Biol Rev [Internet]. 2007 [cited 19 Feb 2021], Vol. 71, (2), pp. 255-281. Available
in: https://mmbr.asm.org/content/mmbr/71/2/255.full.pdf. | spa |
dc.relation.references | Bravo A. Phylogenetic Relationships of Bacillus thuringiensis d-Endotoxin. J. of
Bacteriology [Internet]. 1997 [Cited Feb 19 2021], Vol. 179, (9), pp. 2973-2801. Available in:
https://jb.asm.org/content/179/9/2793. | spa |
dc.relation.references | Del Toro G. Caracterizacion del espectro de acción de la toxina CryAbMod, activa contra
insectos resistentes, y su comparación con la toxina conevncional CryAbMod de Bacillus
thuringiensis [Resis pregrado Biólogo]. México: Universisdad Nacional Autónoma de México;
2010. Disponible en:
https://www.conacyt.gob.mx/cibiogem/images/cibiogem/Fomento-investigacion/Tesis/Caracteriz
acion-espectro-de-accion-toxina-Cry1AbMod.pdf | spa |
dc.relation.references | Chris J. Lacomel, Michelle A, Bradley A. Branching out the aerolysin, ETX/MTX-2 and
Toxin_10 family of pore forming proteins. J.Invertebr.Pathol. [Internet]. 2021 [Cited 24 Aug
2021], págs. 1-9. Available in:
https://www-sciencedirect-com.hemeroteca.lasalle.edu.co/science/article/pii/S002220112100037
9. | spa |
dc.relation.references | Okumura S, Ohba M, Mizuki E, Crickmore N, Côté J.-C, Nagamatsu Y, et al.
Parasporin nomenclature [Internet ]. 2006 [2015; Cited 17 Mar 2021]. Available in:
http://parasporin.fitc.pref.fukuoka.jp/. | spa |
dc.relation.references | Crickmore N, Berry C, Panneerselvam S, Mishra R, Connor T.R. and Bonning B.C.
Bacterial Pesticidal Protein Resource Center [Internet]. Updated [2020; Cited 25 mar2021].
Available in: https://www.bpprc.org/. | spa |
dc.relation.references | [Internet], THE LENS. 2021 [Cited 29 Aug 2021]. Available in:
https://www.lens.org/lens/labs. | spa |
dc.relation.references | Letunic I, Bork P. Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree
display and annotation. Nucleic Acids Research [Interent]. 2021 [Cited 17 Aug 2021], Vol. 48,
págs. W293–W296. Available in: https://academic.oup.com/nar/article/49/W1/W293/6246398 | spa |
dc.relation.references | Madden T. The BLAST Sequence Analysis Tool. The NCBI Handbook [Internet]. 2nd
edition, 2013 [Cited 11 Jun 2021]. Available in:
https://www.ncbi.nlm.nih.gov/books/NBK153387/. | spa |
dc.relation.references | Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden T. NCBI
BLAST: a better web interface. Nucleic Acid Research. 2008 [cited 11 Jun 2021], Vol. 36, págs.
5-9. Available in: https://academic.oup.com/nar. | spa |
dc.relation.references | Uemori A, Ohgushi A,Yasutake K, Maeda M, Mizuki E, OHBA M. Parasporin-1Ab, a
Novel Bacillus thuringiensis Cytotoxin Preferentially Active on Human Cancer Cells In Vitro.
ANTICANCER RESEARCH [Internet]. 2008 [Cited 24 Aug 2021], Vol. 28, págs. 91-96.
Available in: https://ar.iiarjournals.org/content/anticanres/28/1A/91.full.pdf. | spa |
dc.relation.references | Huber, Harald, y otros. The phylum Nanoarchaeota: Present knowledge and future
perspectives of a unique form of life | spa |
dc.relation.references | Ekino K, Okumura S, Ishikawa T, Kitada S, Saitoh H, Akao T, Mizuki E. Cloning and
Characterization of a Unique Cytotoxic Protein Parasporin-5 Produced by Bacillus thuringiensis
A1100 Strain. Toxins [Internet]. 2014 [Cited 24 Aug 2021], Vol. 6, (6), págs. 882–1895.
Available in: https://www.mdpi.com/2072-6651/6/6/1882. | spa |
dc.relation.references | Cole A. R, Gibert M, Popoff M, Moss D. S, Titball R. W, Basak A. Clostridium
perfringens ε-toxin shows structural similarity to the pore-forming toxin aerolysin. Nature
Structural & Molecular Biology [Internet]. 2004 [Cited 24 Aug 2021], Vol. 11, (8), págs.
797–798. Available in: https://www.nature.com/articles/nsmb804. | spa |
dc.relation.references | Berry C, Crickmore N. Structural classification of insecticidal proteins – Towards an in
silico characterisation of novel toxins. J. Invertebrate Pathology [Internet]. 2017[Cited 24 Aug
2021], Vol. 142, págs. 16–22. Available in:
https://www.sciencedirect.com/science/article/abs/pii/S0022201116301008 | spa |
dc.relation.references | Expasy ProtParam tool, [Internet]. Gasteiger E. 1993 [Updated oct 2020]; Cited may 13
2021. Available in: https://web.expasy.org/protparam/. | spa |
dc.relation.references | Ciborowski P, Silberring J. Proteomic Profiling and Analytical Chemistry (2nd edition)
[Internet]. ELSEVIER, 2016 [Cited jul 25 2021]. Available
in:https://www.sciencedirect.com/book/9780444636881/proteomic-profiling-and-analytical-che
mistry. | spa |
dc.relation.references | Bhatia S, Dahiya R. Chapter 4 - Concepts and Techniques of Plant Tissue Culture Science.
Modern Applications of Plant Biotechnology in Pharmaceutical Science. 2015, págs. 121-156.
Available in: https://www.sciencedirect.com/science/article/pii/B9780128022214000042. | spa |
dc.relation.references | Baud F, Karlin S. Measures of residue density in protein structures. PANAS [Internet]. 1999
[Cited jul 25 2021], Vol. 9, (22), págs. 12494–12499. Available in:
https://www.pnas.org/content/96/22/12494. | spa |
dc.relation.references | Katayama H, Kusaka Y, Yokota H, Akao T, Kojima M, Nakamura O, Mizuki E.
Parasporin-1, a Novel Cytotoxic Protein from Bacillus thuringiensis, Induces Ca2+ Influx and a
Sustained Elevation of the Cytoplasmic Ca2+ Concentration in Toxin-sensitive Cells. JBC
[Internet]. 2007 [Cited 24 Aug 2021], Vol. 282, (10), págs. 7742–7752. Available in:
https://www-sciencedirect-com.hemeroteca.lasalle.edu.co/science/article/pii/S002192582063616
X. | spa |
dc.relation.references | Guruprasad K, Reddy B. V. B, Pandit M. W. Correlation between stability of a protein and
its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its
primary sequence. Protein Engineering Design and Selection. 1990 [Cited jul 28 2021], Vol. 4,
(2), págs. 155–161. Available in:
https://academic.oup.com/peds/article-abstract/4/2/155/1491271. | spa |
dc.relation.references | Kyte J, Doolittle F. A simple method for displaying the hydropathic character of a protein.
JMB [Internet]. 1982 [Cited jul 30 2021], Vol. 157, (1), págs. 105-32. Available in:
https://www.sciencedirect.com/science/article/abs/pii/0022283682905150?via%3Dihub. | spa |
dc.relation.references | Gamage D, Gunaratne A, Periyannan G, Russell T. Applicability of Instability Index for
In vitro Protein Stability Prediction. J. Protein & Peptide Letters [Internet]. 2019 [cITED 24
aUG 2021], Vol. 26, (5), págs. 339-347. Available in:
https://www.eurekaselect.com/170322/article. | spa |
dc.relation.references | Jiang L, George S. C. Biomarker signatures of Upper Cretaceous Latrobe Group petroleum
source rocks, Gippsland Basin, Australia: Distribution and geological significance of aromatic
hydrocarbons. Organic Geochemistry [Internet]. 2019 [24 Aug 2021], Vol. 138, págs. 1-20.
Available in:
https://ezproxy.unicolmayor.edu.co:2163/science/article/pii/S014663801930141X?pes=vor. | spa |
dc.relation.references | Moazamian E, Bahador N, Azarpira N, Rasouli M. nti-cancer Parasporin Toxins of New
Bacillus thuringiensis Against Human Colon (HCT-116) and Blood (CCRF-CEM) Cancer Cell
Lines. Current Microbiology [Internet]. 2018 [Cited 24 Aug 2021], Vol. 75, (8), págs.
1090–1098. Available
in:https://ezproxy.unicolmayor.edu.co:2425/article/10.1007/s00284-018-1479-z?utm_source=get
ftr&utm_medium=getftr&utm_campaign=getftr_pilot. | spa |
dc.relation.references | Harada K, Akiba T, Mizushiro H, Ichimatsu T, Katayama H, Akao T, et al. Parasporin-1
Crystal Activated So as to Express Cancer Cell Recognition Destruction Activity, Method for
Producing the Same, Three-Dimensional Structure of the Same, and Method of Using the Same.
Nat Inst of Adv Ind & Technol. JP 2005263728 A 2004. | spa |
dc.relation.references | Li P, Zheng A, Tang Y, Jun Z, Wang S, Deng Q, et al. Bacillus thuringiensis ST7 and
anticancer gene and application thereof. Univ Sichuan Agricultural. CN102517228A 2011. | spa |
dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.lemb | Patentes de parasporina | |
dc.subject.proposal | Bacillus thuringiensis | spa |
dc.subject.proposal | Parasporinas | spa |
dc.subject.proposal | Parámetros bioquímicos | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |